DNA Repair Activity in the Skin of Day and Night Shift Workers
This study is looking at how skin cells repair themselves after being exposed to artificial sunlight (ultraviolet B radiation or UVB). Researchers want to see if there are differences in this repair process between people who work day shifts and those who work night shifts. They will compare how a specific DNA repair factor (XPA) and certain "clock genes" (which control daily body rhythms) are expressed, and how well the skin's natural repair system (nucleotide excision repair or NER) works. To join, you need to be between 18 and 40 years old, have fair skin (Fitzpatrick types I and II), and either primarily work day shifts or night shifts. The study aims to understand if working different shifts affects how your skin responds to UVB.
- Study design
- This is an interventional study with a planned enrollment of 48 participants. It is not specified if it is randomized or blinded.
- What's involved
- You would have one skin punch biopsy taken. This biopsy will then be exposed to ultraviolet B radiation in a laboratory setting.
- Compensation
- Not stated in the trial record.
- Follow-up
- The primary measurements are taken after 1 hour of incubation following ultraviolet B radiation exposure.
AI-generated from the public study record. Only the study team can confirm whether you're eligible — confirm details with them before making decisions.
DNA Repair Activity in the Skin of Day and Night Shift Workers
At a glance
Conditions
NCT04122456
Where you'd take part
This study runs at 1 site. They're the same protocol — you choose where, and that choice sets who your contact draft is addressed to.
Wright State Physicians
Fairborn, Ohiostudy coordinator listed
Recruiting
Sites open and close at different times, so the status above is per site — it can differ from the study's overall status.
Study leadership
- Jeffrey B Travers, MD, PhD · PRINCIPAL_INVESTIGATOR · Wright State University
Who to contact
Opens a ready-to-send draft in your own email app — review before sending.
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Inclusion
Exclusion
What this trial measures
- Expression level of the DNA repair factor XPA at two times of the day in the skin of day and night shift workers.After 1 hour incubation from ultraviolet B radiation exposure.
Reverse transcription quantitative PCR (RT-qPCR) and immunohistochemistry will be used to measure XPA expression at the mRNA and protein level, respectively, in skin samples obtained at 8 am and 4 pm.
- Expression levels of core clock genes at two times of the day in the skin of day and night shift works.After 1 hour incubation from ultraviolet B radiation exposure.
Reverse Transcription Quantitative PCR (RT-qPCR) is used to measure the core circadian clock gene expression at the mRNA level in the skin samples obtained at 8 AM and 4 PM.
- Activity of the nucleotide excision repair (NER) system at two times of the day in day and night shift workers.After 1 hour incubation from ultraviolet B radiation exposure.
A biochemical assay of nucleotide excision repair is used to measure NER in the skin samples obtained at 8 AM and 4 PM.
- Activation of DNA damage kinase signaling pathways at two times of the day in the skin of day and night shift workers.After 1 hour incubation from ultraviolet B radiation exposure.
Immunohistochemistry is used to measure DNA damage kinase signaling (phosphorylated Chk1 and p53) in the skin samples obtained at 8 AM and 4 PM.