Metabolic Adaptations to Weight Loss With and Without Exercise

This study is looking at how your body changes when you lose weight, specifically comparing weight loss with exercise to weight loss without exercise. Researchers want to understand how adding exercise to a weight loss program affects your metabolic health (how your body uses energy) and fat tissue. You might be able to join if you are between 18 and 40 years old, have a Body Mass Index (BMI) between 30 and 40 kg/m2 (which indicates obesity), and don't regularly exercise. The study will measure things like insulin sensitivity (how well your body uses insulin to control blood sugar) and fatty acid turnover (how your body processes fats) to see how these different approaches to weight loss impact your body. The study is currently recruiting 68 participants.

Study design
This is an interventional study that will enroll 68 participants. Participants will be randomly assigned to either a moderate intensity continuous exercise group or a group that remains sedentary.
What's involved
Participants in the exercise group will complete moderate intensity continuous exercise 4 days per week. The study will measure insulin sensitivity and fatty acid turnover at 2 hours, and adipose tissue capillarization (blood vessel formation in fat tissue) at 30 minutes.
Compensation
Not stated in the trial record.
Follow-up
Participants will be followed for measurements taken at 30 minutes and 2 hours after certain procedures.

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NCT05806801

Metabolic Adaptations to Weight Loss With and Without Exercise

Recruiting
NAAges 18–40InterventionalBasic science
University of Michigan
~68 participants
Updated 2026-04-14 on ClinicalTrials.gov
What's tested:MICT ExerciseNo Exercise (Control)

At a glance

Recruiting sites
1 of 1 listed site is recruiting right now
RecruitingSuspended, closed, or not yet open
What they're measuring
Insulin sensitivity
Measured over 2 hours
+3 more outcomes measured
Obesity
Metabolic Disease
Metabolic Syndrome
Insulin Sensitivity
Insulin Resistance
Weight Loss
1 sites across 1 states
Michigan1
  • Jeffrey Horowitz, PhD · PRINCIPAL_INVESTIGATOR · University of Michigan

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Eligibility criteria

Inclusion

Age: 18-40
Body Mass Index: 30-40 kg/m2
Weight stable (±3kg for greater than or equal to about 2 months)
No regularly planned exercise/physical activity
Women must have regularly occurring menses and must be premenopausal

Exclusion

EKG abnormalities
Evidence/history of cardiovascular disease, diabetes or other metabolic disease
Medications known to affect lipid or glucose metabolism
Pregnant or lactating
Tobacco or e-cigarette use
Prior experience of hypersensitivity to insulin, human albumin, and potassium chloride injection.
Allergies/hypersensitivity to local anesthetics of the amide type (e.g., lidocaine)
History of hyperkalemia or potential for developing hyperkalemia (including but not limited to taking drugs that may induce hyperkalemia such as cardiac glycosides or potassium sparing diuretics)
  • Insulin sensitivity2 hours

    a hyperinsulinemic-euglycemic clamp will be used to quantify peripheral insulin sensitivity

  • Fatty acid turnover2 hours

    stable isotope tracer infusion methods will be used to assess fatty acid turnover

  • Adipose tissue capillarization30 minutes

    measured immunohistochemically using an antibody for CD31, quantified using ImageJ software

  • Adipose tissue fibrosis30 minutes

    measured histologically using Sirus Red Staining, quantified using ImageJ software