Microbiome Study for Amputation with Osseointegration

This study is looking at how the community of tiny living things (microbiome) on your skin changes after osseointegration (OI) surgery, a procedure where a prosthetic limb is directly connected to your bone. Researchers will collect skin samples from 50 people who have had OI surgery at different times over 3 to 4 years. They want to see if the surgery causes changes in your skin microbiome and if these changes can help predict how well your wound heals. They will also assess the health of your soft tissue. You can join if you are between 18 and 75 years old and are planning to have OI surgery. The study is currently unclear on its recruitment status.

Study design
This is an observational study involving 50 participants. Researchers will collect samples and data at eight timepoints.
What's involved
You would provide skin swab samples and information about your medications. These procedures will coincide with your regular clinical visits/follow-ups.
Compensation
Not stated in the trial record.
Follow-up
Participants will be followed for up to 48 months (4 years) after the start of the study.

AI-generated from the public study record. Only the study team can confirm whether you're eligible — confirm details with them before making decisions.

NCT06310239

Microbiome Population Adaptation Study

Recruiting
Not specifiedAges 18–75Observational
Walter Reed National Military Medical Center
~50 participants
Updated 2025-08-26 on ClinicalTrials.gov
What's tested:Collection of biologic samples and data

At a glance

Recruiting sites
1 of 1 listed site is recruiting right now
RecruitingSuspended, closed, or not yet open
What they're measuring
Determine if OI surgery causes changes in the microbiome community using phylogenies
Measured over month 36
+2 more outcomes measured
Amputation With Osseointegration

NCT06310239

Where you'd take part

This study runs at 1 site. They're the same protocol — you choose where, and that choice sets who your contact draft is addressed to.

  • Orhopaedic Surgery Service, Walter Reed National Military Medical Center,

    Bethesda, Marylandstudy coordinator listed

    Recruiting

Sites open and close at different times, so the status above is per site — it can differ from the study's overall status.

  • Ean R Saberski, MD · PRINCIPAL_INVESTIGATOR · Walter Reed National Military Medical Center

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Eligibility criteria

Inclusion

Any adult male or female that will undergo an OI procedure between the ages of 18-75.
Defense Enrollment Eligibility Reporting System (DEERs) eligible

Exclusion

Inability to provide self-consent
  • Determine if OI surgery causes changes in the microbiome community using phylogeniesmonth 36

    We will use phylogenies and phylogenetic comparative methods to quantify the changes in the cutaneous microbiome flora before and after OI surgery. This will be done by comparing phylogenies across time points to determine changes in species lineage composition.

  • Determine if changes in the microbiome community predicts wound healing using phylogenetic comparative methodsmonths 36-48

    We will use phylogenetic comparative methods and community turnover rates, quantified via alpha and beta diversity measures, to determine if community perturbations impact wound healing.

  • Quantify soft tissue health using a modified Holger Score (0 = healthy soft issue and 6 = unhealthy soft tissue) to phylogenetically correlate this with microbiome homeostasis.months 36-48

    We will clinically quantify soft tissue stability using a modified Holger Score, then, we will use a phylogenetic correlation to determine if soft tissue health drives microbiome homeostasis