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Melatonin Supplementation and Exercise Recovery in Resistance-Trained Adults

This study is looking at how taking melatonin supplements affects your body's recovery after a tough workout. Researchers want to see if 5mg of melatonin, taken three times a day for 72 hours, can help your cells respond better and improve your recovery after dynamic resistance exercise. You might be able to join if you are a healthy man or woman between 18 and 40 years old, and you regularly do resistance training. The study will measure how you feel regarding your sleep duration, sleep quality, and fatigue to see if melatonin makes a difference. The current status of this study is unclear, and it aims to enroll 24 participants.

Study design
This study is a repeated measures parallel-group investigation, meaning participants will be divided into groups and measured multiple times. It plans to enroll 24 participants.
What's involved
Participants will take either a melatonin gummy or a placebo gummy three times daily for 72 hours, starting 24 hours before and ending 48 hours after a resistance exercise session. You will be asked about your sleep duration, sleep quality, and fatigue at several time points.
Compensation
Not stated in the trial record.
Follow-up
Your sleep duration, sleep quality, and fatigue will be measured up to 48 hours after the exercise session.

AI-generated from the public study record. Only the study team can confirm whether you're eligible — confirm details with them before making decisions.

NCT06617351

This Repeated Measures Parallel-group Investigation Will Examine the Influence of Short-term Melatonin Supplementation (5mg, 3 x Day for 72 Hours) on Cellular Responses, Functional Performance and Recovery Following an Acute Bout of Dynamic Resistance Exercise in Resistance Trained Men and Women

Not Yet Recruiting
NAAges 18–40Interventional
University of Central Florida
~24 participants
Updated 2024-10-01 on ClinicalTrials.gov
What's tested:MelatoninPlacebo

At a glance

Recruiting sites
0 of 1 listed site is recruiting right now
RecruitingSuspended, closed, or not yet open
What they're measuring
Subjective Sleep Duration
Measured over Pre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)
+50 more outcomes measured

Conditions

Cell Migration
Cell Invasion
Exercise Recovery

Explore related trials

Healthy Volunteers

NCT06617351

Where you'd take part

This study runs at 1 site. They're the same protocol — you choose where, and that choice sets who your contact draft is addressed to.

  • Kinesiology Research Labs

    Orlando, Floridano site contact published

Sites open and close at different times, so the status above is per site — it can differ from the study's overall status.

Who to contact

Adam J Wells, PhD
[email protected]
407-823-3906
Email the study team

Opens a ready-to-send draft in your own email app — review before sending.

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Eligibility criteria

Inclusion

Male or female between the ages of 18-40 years old.
Healthy and ready for physical activity as determined by the Physical Activity Readiness Questionnaire (PAR-Q+) and Medical History Questionnaire (MHQ).
Active resistance training for a minimum of 6 months as defined by 3 resistance training sessions (muscle strengthening activities such as free weights, weight machines, or calisthenics, etc.) per week with at least one lower body session as determined by the training history questionnaire.
Premenopausal, with an identifiable onset of menses (early follicular phase) as determined by the menstrual status questionnaire (female participants only)..
Not currently pregnant and no intention to become pregnant for the duration of participation (female participants only).
Currently free from and willing to abstain from dietary supplements that are viewed by study investigators to confound the outcomes of the study (e.g., creatine, beta-alanine) for the duration of the study. Participants currently using supplements will be permitted to enroll in the study following a 4-week washout period of these supplements.
Willing to adhere to all pre-testing visit instructions including abstaining from exercise for the duration of the study and abstaining from alcohol for 24 hours prior to visit 2 as well as for 24 hours before visit 3 until completion of the study.
Currently be consuming ≤ 300mg caffeine per day on average and willing to keep caffeine intake consistent throughout the duration of the study. Participants must also be willing to abstain from caffeine intake for 12 hours prior to visits 3, 4 and 5 and not consume caffeine the morning of these visits.
Free from previous or current lower body injuries that are viewed by the investigators to potentially limit ability of the participant to perform the exercise intervention or functional assessments.
Not regularly taking any type of prescription or over-the-counter medication which might affect the assessments, or having any chronic illnesses, which require medical care.
Considered by the study investigators to have a high likelihood of successful venipuncture following an initial examination of the participants antecubital fossa by a certified phlebotomist and verbal discussion of the participants blood draw history (including history of unsuccessful venipunctures, known issues with the locating/palpating of veins in the antecubital fossa by a phlebotomist, and whether these difficulties are more apparent on one arm compared to the other).
Currently taking melatonin
Regularly taking any type of prescription or over-the-counter medication which might affect the assessments, or having any chronic illnesses, which require medical care.
Not currently meeting requirements for resistance trained status.
Current known pregnancy or intent to become pregnant during the study period.
Not regularly having periods or amenorrheic, as determined by Menstrual Status Questionnaire (MSQ).
Currently taking any performance-enhancing drug (determined from health and activity questionnaire).
Currently taking a nutritional supplement viewed by the research team to confound the outcomes of the study and not willing to abstain from taking the supplement during the course of the study or not willing to undergo a 4-week wash-out period prior to participating if required.
Evaluated as having a low likelihood of successful venipuncture by a certified phlebotomist.

Exclusion

Individual does not agree to participate in this study.

What this trial measures

  • Subjective Sleep DurationPre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Participants provide information about sleep duration the previous night using the Hooper Questionnaire administered via pen and paper. Response is rated on a seven-point Likert scale with the following responses: * 10+ * 9-10 * 8-9 * 8 * 7-8 * 5-7 * 5 or less

  • Subjective Sleep QualityPre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Participants provide information about their sleep quality the previous night using the Hooper Questionnaire administered via pen and paper. Response is rated on a seven-point Likert scale with the following responses: * Outstanding * Very good * Good * Better than normal * Worse than normal * Disrupted * Horrible - No Sleep

  • Subjective FatiguePre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Participants provide information about their current level of fatigue using the Hooper Questionnaire administered via pen and paper. Response is rated on a seven-point Likert scale with the following responses: * No Fatigue * Minimal Fatigue * Better than normal * Normal * Worse than normal * Very Fatigued * Exhausted - Major fatigue

  • Subjective Muscle SorenessPre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Participants provide information about their current level of muscle soreness using the Hooper Questionnaire administered via pen and paper. Response is rated on a seven-point Likert scale with the following responses: * No Soreness * Very little soreness * Better than normal * Normal * Worse than normal * Very sore/tight * Extremely sore/tight

  • Subjective Mental StressPre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Participants provide information about their current level of mental stress using the Hooper Questionnaire administered via pen and paper. Response is rated on a seven-point Likert scale with the following responses: * Feeling great - Very relaxed * Feeling Good - Relaxed * Better than normal * Normal * Worse than normal * Stressed * Very Stressed

  • Perceived Recovery StatusPre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Participants provide information about their perceived recovery status using the Perceived Recovery Status Scale (PRSS). Response is rated on a scale ranging from 0 (very poorly recovered/extremely tired) to 10 (very well recovered/highly energetic). Each response also corresponds to a broader performance categorization with values indicating expectations of either declined performance, similar performance, or improved performance relative to subsequent time points.

  • Active Range of Motion (AROM)Pre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Assessed in both lower limbs via handheld goniometer while participant is supine. Average of two measures on each limb is used to determine AROM.

  • Pain Pressure Threshold (PPT)Pre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Assessed in dominant lower limb via manual algometer while participant is supine. PPT is defined as the point at which the pressure applied via the algometer becomes uncomfortable, as verbally indicated by the participant. PPT is measured in triplicate with 10 seconds of rest between measurements. Average of the three measures is used to determine PPT.

  • Peak Jump HeightPre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Determined via counter movement jump performed using portable force plate system. Peak Jump height is the highest jump height across 3 jumps separated by 30 seconds.

  • Mean Jump HeightPre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Determined via counter movement jump performed using portable force plate system. Mean Jump height is the average jump height across 3 jumps separated by 30 seconds.

  • Peak Jump ForcePre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Determined via counter movement jump performed using portable force plate system. Peak force is the highest force achieved across 3 jumps separated by 30 seconds.

  • Mean Jump ForcePre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Determined via counter movement jump performed using portable force plate system. Mean Jump force is the average jump force across 3 jumps separated by 30 seconds.

  • Peak Braking ForcePre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Determined via counter movement jump performed using portable force plate system. Peak braking force is the highest braking force achieved across 3 jumps separated by 30 seconds.

  • Mean Braking ForcePre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Determined via counter movement jump performed using portable force plate system. Mean braking force is the average braking force across 3 jumps separated by 30 seconds.

  • Peak Braking Rate of Force Development (RFD)Pre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Determined via counter movement jump performed using portable force plate system. Peak braking rate of force development force is the peak braking RFD force achieved across 3 jumps separated by 30 seconds.

  • Mean Braking Rate of Force Development (RFD)Pre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Determined via counter movement jump performed using portable force plate system. Mean braking rate of force development is the average braking RFD force across 3 jumps separated by 30 seconds.

  • Mean Reactive Strength Index (RSI)Pre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Determined via counter movement jump performed using portable force plate system. Defined as the time taken to complete the flight phase divided by the total time taken from the initiation of movement to the instant of take-off. Mean RSI is the average RSI across 3 jumps separated by 30 seconds.

  • Peak Reactive Strength Index (RSI)Pre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Determined via counter movement jump performed using portable force plate system. Defined as the time taken to complete the flight phase divided by the total time taken from the initiation of movement to the instant of take-off. Peak RSI is the peak RSI achieved across 3 jumps separated by 30 seconds.

  • Squat Maximal Voluntary Isometric Contraction (MVIC) ForcePre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Determined via isometric squat contraction using portable force plate system and isometric squat rack with fixed bar. Participants will complete three 5-second isometric contractions with one minute of rest between contractions. The highest force achieved during the three contractions will be recorded as Squat MVIC force.

  • Squat Maximal Voluntary Isometric Contraction (MVIC) Rate of Force Development (RFD)Pre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Determined via isometric squat contraction using portable force plate system and isometric squat rack with fixed bar. Participants will complete three 5-second isometric contractions with one minute of rest between contractions. Rate of force development (RFD), dictated by the onset of muscular contraction, will be recorded at 0-300ms for the contraction with the highest peak force.

  • Objective Sleep Quality-24 hours before (-24H), 24-hours Post (24H), 48-hours Post (48H)

    Assessed using an ActiGraph GT9X Link accelerometer. Participants will wear the accelerometer and beginning 24 hours before the resistance exercise protocol through 48-hours post-exercise. Number of minutes that participants are asleep, and the number of times their sleep is disrupted will be assessed.

  • Physical Activity-24 hours before (-24H), 24-hours Post (24H), 48-hours Post (48H)

    Assessed using an ActiGraph GT9X Link accelerometer. Participants will wear the accelerometer and beginning 24 hours before the resistance exercise protocol through 48-hours post-exercise. Minutes per day that participants engage in low, moderate, and high intensity physical activity will be assessed.

  • HematocritPre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Assessed via Automated Hematology Analyzer

  • HemoglobinPre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Assessed via Automated Hematology Analyzer

  • Mean corpuscular volume (MCV)Pre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Assessed via Automated Hematology Analyzer

  • Mean corpuscular hemoglobin (MCH)Pre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Assessed via Automated Hematology Analyzer

  • Mean corpuscular hemoglobin concentration (MCHC)Pre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Assessed via Automated Hematology Analyzer

  • Total white blood cell count (WBC)Pre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Assessed via Automated Hematology Analyzer

  • Total red blood cell count (RBC)Pre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Assessed via Automated Hematology Analyzer

  • Lymphocyte CountPre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Assessed via Automated Hematology Analyzer

  • Monocyte CountPre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Assessed via Automated Hematology Analyzer

  • Granulocyte CountPre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Assessed via Automated Hematology Analyzer

  • Platelet CountPre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Assessed via Automated Hematology Analyzer

  • Serum Creatine Kinase (CK) concentrationPre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Marker of muscle damage. Assessed via assay and microplate reader

  • Serum Melatonin concentrationPre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Assessed via assay and microplate reader

  • Serum C-reactive protein (CRP) concentrationPre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Assessed via assay and microplate reader

  • Serum Interleukin-8 (IL-8) concentrationPre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Assessed via assay and microplate reader

  • Serum Monocyte Chemoattractant Protein-1 (MCP-1) concentrationPre (0), Immediately-post (IP), 4-hours Post (4H), 24-hours post (24H), 48-hours post (48H)

    Assessed via assay and microplate reader

  • Monocyte Cell Index (CI)Pre (0), 24-hours post (24H), 48-hours post (48H)

    Assessed via Real Time Cell Analysis (RTCA) assay

  • Neutrophil Cell Index (CI)Pre (0), 4-Hours Post (4H), 24-hours post (24H)

    Assessed via Real Time Cell Analysis (RTCA) assay

  • Monocyte C-C chemokine receptor 2 (CCR2) expressionPre (0), 24-hours post (24H), 48-hours post (48H)

    Assessed via flow cytometry

  • Monocyte Macrophage-1 antigen (Integrin CD11b) expressionPre (0), 24-hours post (24H), 48-hours post (48H)

    Assessed via flow cytometry

  • Monocyte Melatonin Receptor 1A (MLTr1A) expressionPre (0), 24-hours post (24H), 48-hours post (48H)

    Assessed via flow cytometry

  • Neutrophil Receptor 1A (MLTr1A) expressionPre (0), 4-Hours Post (4H), 24-hours post (24H)

    Assessed via flow cytometry

  • Neutrophil Macrophage-1 antigen (Integrin CD11b) expressionPre (0), 4-Hours Post (4H), 24-hours post (24H)

    Assessed via flow cytometry

  • Neutrophil Interleukin-8 receptor (CXCR2) expressionPre (0), 4-Hours Post (4H), 24-hours post (24H)

    Assessed via flow cytometry

  • Diet - Daily ProteinPre (0), 24-hours Post (24H), 48-hours Post (48H)

    Assessed via Automated Self-Administered 24hr Dietary Recall (ASA24).

  • Diet - Daily CarbohydratePre (0), 24-hours Post (24H), 48-hours Post (48H)

    Assessed via Automated Self-Administered 24hr Dietary Recall (ASA24).

  • Diet - Daily FatPre (0), 24-hours Post (24H), 48-hours Post (48H)

    Assessed via Automated Self-Administered 24hr Dietary Recall (ASA24).

  • Diet - Daily Total CaloriesPre (0), 24-hours Post (24H), 48-hours Post (48H)

    Assessed via Automated Self-Administered 24hr Dietary Recall (ASA24).

  • Diet - Daily Total MicronutrientsPre (0), 24-hours Post (24H), 48-hours Post (48H)

    Assessed via Automated Self-Administered 24hr Dietary Recall (ASA24). Participants report each eating occasion and the time of consumption during a 24-hour period.