Nutrient Profile and Muscle Protein Synthesis in Response to Corn Bread
This study is looking at how different types of corn bread affect your body. Specifically, it compares corn bread made from refined maize flour versus whole grain maize flour. Researchers want to see how these different flours impact the nutrients and hormones in your blood, and how they affect muscle protein synthesis (the process your body uses to build and repair muscles). They will measure these changes in your blood for up to 3 hours after you eat. This study is for healthy, active men and women between 18 and 30 years old. The goal is to understand which type of corn bread might be better for muscle health. The study plans to enroll 20 participants, but its current status is unclear.
- Study design
- This is an interventional study involving 20 participants, comparing meals made with refined maize flour versus whole grain maize flour.
- What's involved
- You would consume meals made from either refined maize flour or whole grain maize flour. Blood samples will be taken at various times for up to 3 hours after eating.
- Compensation
- Not stated in the trial record.
- Follow-up
- Your blood will be monitored for up to 180 minutes (3 hours) after consuming the meal.
AI-generated from the public study record. Only the study team can confirm whether you're eligible — confirm details with them before making decisions.
Nutrient Profile and Muscle Protein Synthesis in Response to Corn Bread Made From Refined Versus Whole Grain Maize Flour
At a glance
Conditions
Where it's being run
1 sites across 1 statesStudy leadership
- Keith Baar · PRINCIPAL_INVESTIGATOR · University of California, Davis
Who to contact
This trial hasn't published a contact. View it on ClinicalTrials.gov
Do you actually qualify for this trial?
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Exclusion
What this trial measures
- post-prandial amino acid profile[Time Frame: Baseline (0 hour) to 30, 60, 90, 120 and 180 minutes after consumed meal]
Serum samples are extracted with 1mL of modified Folch extraction, followed by vacuum centrifugation for drying. Dried samples will be reconstituted with 100 μl of 75% ACN/water. The supernatant is then injected into Aligent UPLC-QqQ for analysis of targeted free amino acids, short peptides, and other metabolites.
- post-prandial short peptide profile[Time Frame: Baseline (0 hour) to 30, 60, 90, 120 and 180 minutes after consumed meal]
Serum samples are extracted with 1mL of modified Folch extraction, followed by vacuum centrifugation for drying. Dried samples will be reconstituted with 100 μl of 75% ACN/water. The supernatant is then injected into Aligent UPLC-QqQ for analysis of targeted free amino acids, short peptides, and other metabolites.
- Muscle protein synthesis[Time Frame: Baseline (0 hour) to 30, 60, 90, 120 and 180 minutes after consumed meal]
To measure muscle protein synthesis, stably transfected C2C12 muscle cells with a plasmid (pcDNA3 luciferase) will be used. C2C12Luc cells will be plated in 24-well plates and differentiated over 4 days. Differentiated C2C12 cells will be fasted by washing with PBS and then treating them with Test Media (20% DMEM) for 15 minutes. Fasted muscle cells will then be treated with Test Media containing 10% baseline or fed serum (from blood samples at 30, 60, 90,120 and 180 minutes after meal) for 3 hours. Cells will be collected in passive lysis buffer and firefly luciferase activity will be determined.
- mTORC1 specific protein synthesis[Time Frame: Baseline (0 hour) to 30, 60, 90, 120 and 180 minutes after consumed meal]
To measure the ability of meal of chicken (conventional or regenerative) to activate mTORC1, stably transfected C2C12 muscle cells with a plasmid (pcDNA3-TOP luciferase) where the luciferase mRNA contains a 5'TOP. 5'TOP mRNA, which specifically regulated by mTORC1 activity, will be used. Differentiated C2C12TOPLuc muscle cells in 24-well plates will be stimulated using the baseline or fed serum (from blood samples at 30, 60, 90,120 and 180 minutes after meal). The degree of mTORC1 activation will be determined as the difference in slopes between the baseline and fed sera.