Dehydration, Rehydration, and Impact on Physiology Study
This study is looking at how changes in your body's hydration (water) levels affect inflammation and overall physical performance in adults aged 45-65. Researchers will use a special 12-day Hydration-Dehydration-Rehydration Protocol (HDR) to carefully change your fluid intake. They will then measure things like your fasting blood sugar and total body water to see these effects. The goal is to understand if mild dehydration, followed by rehydration, influences important body processes like inflammation and how well your body functions. You may be able to join if you are generally healthy, between 45 and 65 years old, and meet certain weight and health requirements. The study is currently recruiting 15 participants, but its exact status is unclear.
- Study design
- This is an interventional study with 15 participants. It involves a specific sequence of hydration, dehydration, and rehydration.
- What's involved
- You will complete a 4-day hydration protocol, followed by a 4-day dehydration protocol, and then a 4-day rehydration protocol, totaling 12 days. Fasting blood glucose and body water measurements will be taken on specific days.
- Compensation
- Not stated in the trial record.
- Follow-up
- Measurements for primary endpoints are taken on Day 2, Day 3, Day 4, and Day 5 of the protocol.
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Dehydration, Rehydration, and Impact on Physiology
At a glance
Conditions
Where it's being run
1 sites across 1 statesWho to contact
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What this trial measures
- Fasting blood glucose (mg/dL)Day 2 (Pre-hydration testing), Day 3 (Pre-dehydration testing), Day 4 (Post-dehydration testing), Day 5 (Post-rehydration testing)
Fasting blood glucose will be measured via fingerstick at each testing visit. Values will be used to examine changes in glucose regulation in response to hydration status across the HDR Protocol phases.
- Total body water (L)Day 2 (Pre-hydration testing), Day 3 (Pre-dehydration testing), Day 4 (Post-dehydration testing), Day 5 (Post-rehydration testing)
Bioelectrical impedance spectroscopy (BIS) will be used to assess changes in total body water, BIS measurements will be collected at each testing visit to evaluate fluid shifts.
- Intracellular water (L)Time Frame: Day 2 (Pre-hydration testing), Day 3 (Pre-dehydration testing), Day 4 (Post-dehydration testing), Day 5 (Post-rehydration testing)
Description: Bioelectrical impedance spectroscopy (BIS) will be used to assess changes in intracellular water across hydration phases. BIS measurements will be collected at each testing visit to evaluate fluid shifts.
- Extracellular water (L)Time Frame: Day 2 (Pre-hydration testing), Day 3 (Pre-dehydration testing), Day 4 (Post-dehydration testing), Day 5 (Post-rehydration testing)
Description: Bioelectrical impedance spectroscopy (BIS) will be used to assess changes in extracellular water across hydration phases. BIS measurements will be collected at each testing visit to evaluate fluid shifts.
- Hand grip strength (kg)Day 2 (Pre-hydration testing), Day 3 (Pre-dehydration testing), Day 4 (Post-dehydration testing), Day 5 (Post-rehydration testing)
Handgrip strength will be measured using a handheld dynamometer at four time points during the HDR Protocol. Measurements will be used to assess the impact of hydration status on upper body muscular strength.
- Isometric leg pull strength (kg)Day 2 (Pre-hydration testing), Day 3 (Pre-dehydration testing), Day 4 (Post-dehydration testing), Day 5 (Post-rehydration testing)
Isometric lower body strength will be evaluated using a standardized leg pull test at four timepoints during the HDR Protocol. Results will be used to determine the effect of hydration status on lower body muscular strength.
- Total sleep time (hrs)through study completion, 18 days
Total sleep time will be continuously monitored throughout the HDR Protocol using a wearable device.
- Sleep efficiency (%)through study completion, 18 days
Sleep efficiency will be continuously monitored throughout the HDR Protocol using a wearable device.
- Number of awakenings (count)through study completion, 18 days
Number of awakenings will be continuously monitored throughout the HDR Protocol using a wearable device.
- PBMC H2O2 ( µg/mL)Day 2 (Pre-hydration testing), Day 3 (Pre-dehydration testing), Day 4 (Post-dehydration testing), Day 5 (Post-rehydration testing)
Peripheral blood mononuclear cells (PBMCs) will be isolated from whole blood samples collected at four time points during the HDR Protocol. H2O2 will be assessed.
- DRP1 (Arbitrary Units)Day 2 (Pre-hydration testing), Day 3 (Pre-dehydration testing), Day 4 (Post-dehydration testing), Day 5 (Post-rehydration testing)
Peripheral blood mononuclear cells (PBMCs) will be isolated from whole blood samples collected at four time points during the HDR Protocol. Fission expression (DRP1).
- MFN (Arbitrary Units)Day 2 (Pre-hydration testing), Day 3 (Pre-dehydration testing), Day 4 (Post-dehydration testing), Day 5 (Post-rehydration testing)
Peripheral blood mononuclear cells (PBMCs) will be isolated from whole blood samples collected at four time points during the HDR Protocol. Mitochondrial fusion expression (MFN) will be assessed.
- PINK1 (Arbitrary Units)Day 2 (Pre-hydration testing), Day 3 (Pre-dehydration testing), Day 4 (Post-dehydration testing), Day 5 (Post-rehydration testing)
Peripheral blood mononuclear cells (PBMCs) will be isolated from whole blood samples collected at four time points during the HDR Protocol. Mitophagy marker (PINK1) will be assessed.
- Parkin (Arbitrary Units)Day 2 (Pre-hydration testing), Day 3 (Pre-dehydration testing), Day 4 (Post-dehydration testing), Day 5 (Post-rehydration testing)
Peripheral blood mononuclear cells (PBMCs) will be isolated from whole blood samples collected at four time points during the HDR Protocol. Mitophagy marker (Parkin) will be assessed.
- Malondialdehyde (nmol/mg)Day 2 (Pre-hydration testing), Day 3 (Pre-dehydration testing), Day 4 (Post-dehydration testing), Day 5 (Post-rehydration testing)
Peripheral blood mononuclear cells (PBMCs) will be isolated from whole blood samples collected at four time points during the HDR Protocol. Lipid peroxidation will be assessed by measuring malondialdehyde concentrations.