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Study on Hydration and Energy Use During Exercise

This study is looking at how your hydration (how much water your body has) affects how your body uses fat and carbohydrates for energy, both at rest and during exercise. Researchers want to understand if different hydration levels change your body's ability to switch between these energy sources, which is called metabolic flexibility. This is important because problems with metabolic flexibility might be linked to conditions like type 2 diabetes. You might be able to join if you are between 18 and 35 years old. The study will measure your Respiratory Exchange Ratio (how much carbon dioxide you breathe out compared to how much oxygen you breathe in) during exercise to see how your body is using energy. The current recruitment status is unclear.

Study design
This interventional study plans to enroll 74 participants. It involves three different hydration plans (DEH, EUH-H2O, and EUH-Electrolyte arms).
What's involved
You would participate in an initial exercise trial, followed by a 24-hour hydration plan, then indirect calorimetry and two cycling sessions. Females will have 13 visits, and males will have 7 visits.
Compensation
Not stated in the trial record.
Follow-up
Your Respiratory Exchange Ratio will be measured over the course of exercise bouts performed during the exercise and experimental trials.

AI-generated from the public study record. Only the study team can confirm whether you're eligible — confirm details with them before making decisions.

NCT07298863

The Effect of Hydration Status on Substrate Oxidation at Rest and During Light and Moderate Exercise

Active, Not Recruiting
NAAges 18–35Interventional
PepsiCo Global R&D
~104 participants
Updated 2026-08-27 on ClinicalTrials.gov
What's tested:Part 1 - Exercise TrialPart 1 - Experimental TrialPart 2 - Oral Glucose Tolerance Test

At a glance

Recruiting sites
0 of 2 listed sites are recruiting right now
RecruitingSuspended, closed, or not yet open
What they're measuring
Respiratory Exchange Ratio
Measured over Measured over the course of exercise bouts performed during the exercise and experimental trials. Compared across DEH, EUH-H2O, and EUH-Electrolyte arms.
+1 more outcome measured

Conditions

Hydration Status
Substrate Metabolism During Exercise

Explore related trials

Hydration statusMetabolic Flexibility

Where it's being run

2 sites across 2 states
New York1
Ontario1

Who to contact

This trial hasn't published a contact. View it on ClinicalTrials.gov

Do you actually qualify for this trial?

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Eligibility criteria

Inclusion

Subject is male or female
If female, subject is not pregnant
If female, subject has stopped taking birth control medication or removed long-acting reversible contraceptive \>6 months at start of the study
If female, subject has normal menstrual cycle (25-35 days)
If female, subject is currently tracking their menstrual cycle using a smartphone application
If female subject is taking iron supplement, they are willing to continue supplementation during entire study
If female, subject has a smartphone compatible for application to track menstrual cycle
Subject is 18-35 years of age, inclusive
Subject body mass index (BMI) is between 18.5-25 kg/m2
Subject is at least recreationally active (engaged in moderate-intensity, intermittent, or steady-state exercise at least 3 days per week for at least 60 min at a time)
Subject is accustomed to cycling for at least 60 min (Part 1 only)
Subject does not smoke (or has quit for at least 6 months)
Subject is not taking medication that may interfere with the study (e.g., diuretics, laxatives, or weight loss drugs)
Subject has no health conditions that would interfere with the study
As indicated on the general health questionnaire (GHQ)
e.g. cardiovascular, renal, or metabolic diseases
Subject is willing to refrain from having MRI scan during study protocol (Part 2 only)
Subject is not actively involved in weight loss intervention (dieting and/or anti-obesity medications)
Subject is not allergic to adhesives (e.g., medical tape)
Subject is not allergic to citrus-raspberry flavor (Part 2 only)
Subject is willing to avoid alcohol consumption 24 hours prior to visit(s)
Subject is willing to fast overnight (\~8-12 hours)
Subject is willing to refrain from vigorous exercise for 24 hours (light physical activity only)
Subject is willing to be shaved at patch or electrode site, if necessary (Part 1 only)
Subject is willing to eat the exact same food prior to each visit to the laboratory
Subject is willing to replicate exact diet and physical activity 24 hours before Visits 2/4 and for the 3 days between Visits 3/5 (Part 2 only)
Able to speak, write, and read English
Provision of written consent to participate

Exclusion

Subject has participated in a clinical trial within the past 30 days
Subject has participated in any PepsiCo trial within past 3 months (with the exception of Part 1 participants)
Subject has a condition or is taking medication that the investigator believes would interfere with his/her ability to provide informed consent, comply with the study protocol, which might confound the interpretation of the study results, or put the person at undue risk
Subject is allergic to alcohol (alcohol prep pads are used for blood draws).
If female, subject is taking birth control medication or has long-acting reversible contraceptive
If female, subject is anemic
If male, subject is undergoing hormone therapy to alter testosterone levels (injections, transdermal patch, pill, etc.)
Subject is employed by, or has a parent, guardian, or other immediate family member employed by a company that manufactures any products that compete with any Gatorade product. If subject is unsure if a company would be considered a competitor to Gatorade, they will be asked to please let the study investigator know the name of the other company and the nature of their relationship to that company before they sign the informed consent.

What this trial measures

  • Respiratory Exchange RatioMeasured over the course of exercise bouts performed during the exercise and experimental trials. Compared across DEH, EUH-H2O, and EUH-Electrolyte arms.

    Part 1 only. VCO2 / VO2 measured using indirect calorimetry measured by metabolic cart (Moxus or similar device) while exercising on cycle ergometer.

  • Blood glucose levelsbaseline and 120-minutes following glucose ingestion for both the dehydration and euhydrated interventions

    Part 2 only. mg/dL