Flourish: Exploring the Early Infant Gut Microbiome

This study, called Flourish, is looking at whether analyzing an infant's gut microbiome (the community of tiny living things in their intestines), providing education, and offering personalized recommendations can improve gut health in babies. It focuses on infants aged 0 to 3 months who were born by C-section. The goal is to see if these interventions can increase helpful bacteria, reduce bacteria linked to C-sections, decrease harmful bacteria, and improve how well the gut can digest certain sugars and produce beneficial substances. Researchers also want to know if these gut changes lead to fewer early signs of conditions like eczema. The study is currently recruiting about 250 infants.

Study design
This is an interventional study planning to enroll 250 participants. It explores the effects of tailored recommendations, consult calls, and an educational email series.
What's involved
Not specified in the trial record.
Compensation
Not stated in the trial record.
Follow-up
Participants' microbiota composition, C-section-associated microbiome signatures, and opportunistic microbial abundance will be measured at 24 months.

AI-generated from the public study record. Only the study team can confirm whether you're eligible — confirm details with them before making decisions.

NCT07333482

Flourish: Exploring the Early Infant Gut Microbiome

Recruiting
NAAges 0–3InterventionalPrevention
Seeding Inc
~250 participants
Updated 2026-02-24 on ClinicalTrials.gov
What's tested:Tailored RecommendationsConsult CallEducational Email Series

At a glance

Recruiting sites
1 of 1 listed site is recruiting right now
RecruitingSuspended, closed, or not yet open
What they're measuring
Microbiota Composition
Measured over 24 months
+4 more outcomes measured
Microbiota
Gut Microbiome
Eczema
Microbiome

NCT07333482

Where you'd take part

This study runs at 1 site. They're the same protocol — you choose where, and that choice sets who your contact draft is addressed to.

  • Seeding Labs INC

    Austin, Texasstudy coordinator listed

    Recruiting

Sites open and close at different times, so the status above is per site — it can differ from the study's overall status.

  • Qian Yuan, MD · PRINCIPAL_INVESTIGATOR · Massachusetts General Hospital

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Eligibility criteria

Inclusion

Infants are qualified for this study if they are 0 to 3 months of age at time of enrollment.
Infants must have been delivered via Cesarean delivery (C-section), either scheduled or emergent.
Infants must have been at least 36 weeks gestation at time of delivery.
Infants and their caregivers must reside in the United States with a US mailing address.

Exclusion

Infants can not have been given probiotic supplements in their life at recruitment. This includes probiotic powder or supplements or formula with probiotic addition or multivitamin with probiotic addition.
Twin and multiple birth infants are not accepted in this study.
Infants cannot have the following existing health conditions:
Gastrointestinal conditions: Hirschsprung disease, eosinophilic gastrointestinal disorders (EGID) including eosinophilic esophagitis (EoE), necrotizing enterocolitis (NEC), short bowel syndrome (SBS)
Immune or auto-immune conditions: Severe Combined Immunodeficiency (SCID), human immunodeficiency virus (HIV)), excluding eczema and rashes
Congenital conditions: cleft lip or cleft palate, congenital heart disease, cerebral palsy, fragile X syndrome, down syndrome, spina bifida, cystic fibrosis, phenylketonuria (PKU), congenital hypothyroidism (CHT), galactosaemia)
Blood disorders (sickle cell disease, thalassemia, hemophilia)
Infant or any immediate family member has previously received results from an at-home microbiome stool test (excluding standard clinical diagnostic testing such as stool culture or pathogen testing)
  • Microbiota Composition24 months

    Changes in gut microbiota composition, including relative abundance of beneficial bacterial taxa such as Bifidobacterium, assessed using shotgun metagenomic sequencing.

  • C-Section-Associated Microbiome Signatures24 months

    Changes in the relative abundance of microbial taxa and signatures previously associated with Cesarean delivery, assessed longitudinally using shotgun metagenomic sequencing.

  • Opportunistic and Potentially Pathogenic Microbial Abundance24 months

    Changes in the relative abundance of opportunistic and potentially pathogenic microbial taxa over time, assessed using shotgun metagenomic sequencing.

  • Functional Microbiome Capacity24 months

    Changes in microbial functional gene pathways related to human milk oligosaccharide (HMO) digestion and short-chain fatty acid (SCFA) production, assessed using metagenomic functional profiling.

  • Eczema Incidence / Atopic Symptoms24 months

    Incidence of eczema and other early atopic symptoms assessed via parent-reported surveys and standardized eczema assessment when symptoms are reported.