Pilot Study: Blocking Injury-Induced MVP Release in Skin Using Topical Amitriptyline
This study is investigating how a small burn injury affects tiny particles in your skin called microvesicle particles (MVPs). Researchers believe MVPs are involved in how skin responds to injuries like burns. They want to see if applying a medication called amitriptyline to the skin can prevent these MVPs from increasing after a burn. You might be able to join if you are an adult between 18 and 64 years old with any skin type, and do not have conditions that affect wound healing or a history of abnormal scarring. The study will compare amitriptyline to a placebo (an inactive substance). The main goal is to see if a burn injury increases MVPs in the skin. The current recruitment status is unclear.
- Study design
- This is an interventional study with a planned enrollment of 50 participants. It will compare the effects of 10% amitriptyline to a placebo.
- What's involved
- You would receive a small burn injury, and then either amitriptyline or a placebo would be applied. Skin biopsies would be taken to measure MVPs.
- Compensation
- Not stated in the trial record.
- Follow-up
- MVPs will be measured at 2 hours after the burn injury.
AI-generated from the public study record. Only the study team can confirm whether you're eligible — confirm details with them before making decisions.
Pilot Studies Blocking Injury-Induced MVP Release in Skin Using Topical Amitriptyline
At a glance
Conditions
Where it's being run
1 sites across 1 statesWho to contact
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Inclusion
Exclusion
What this trial measures
- Change in the amount of microvesicle particles generated in skin from localized thermal burn injury over untreated skin.2 hours after the burn injury
Anesthetized volar forearm skin will be treated with a heated 3.5 mm metal rod to generate a thermal burn injury. Immediately after the burn injury 0.1mL of polyethylene glycol vehicle will be applied. Vehicle will also be applied to nearby un-burned skin. Skin biopsies of burned provided vehicle and nearby unburned area with vehicle will be obtained 2 hours after localized burn injury. MVP will be measured. Briefly, the biopsies will be weighed, and treated with enzymes to break down the tissue. Following several centrifugation steps, the MVP fraction will be measured using a Nanosight instrument. The MVPs will be normalized to biopsy weight. The numbers of MVP in untreated skin will be compared to skin which underwent a thermal burn injury.